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KMID : 0366119860140020139
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Korean Journal of Applied Microbiology & Bioengineering
1986 Volume.14 No. 2 p.139 ~ p.139
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Purification and enzyme characteristics of laccas from Ganoderma lucidum
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Abstract
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The production media and enzymatic characteristics of laccase from Ganoderma lucidum was investigated. Potato dextrose yeast extract media was proved to be the best for laccase production. The enzyme has optimum pH of 6.45, Km value of 6.71 mM and appeared to be stable at wide pH range. The enzyme was inactivated partially by methanol and ethanol and totally by sodium azide but not at all by acetone. Also the enzyme purification was performed and the data is given.
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